ISO 13629 纺织品 抗真菌活性测定

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ISO 13629-1:2012 Textiles -- Determination of antifungal activity of textile products -- Part 1: Luminescence method
 
ISO 13629-1:2012 specifies a test method for the quantitative determination of the antifungal activity by measuring the intensity of luminescence produced by an enzymatic reaction [adenosine triphosphate (ATP) method].
ISO 13629-1:2012 is applicable to various kinds of textile products, such as fibres, yarns, fabrics, clothing, bedclothes, home furnishings and other miscellaneous goods.
 
General information 
Status :  Published
Publication date : 2012-08
Edition : 1
Number of pages : 19
Technical Committee : ISO/TC 38 Textiles
ICS : 59.080.01 Textiles in general
 
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 13629-1 was prepared by Technical Committee ISO/TC 38, Textiles.
ISO 13629 consists of the following parts, under the general title Textiles — Determination of antifungal activity of textile products:
— Part 1: Luminescence method
— Part 2: Plate count method (under preparation)
 
Introduction
Speciality products of antimicrobial-treated textiles have been increasing year by year in various applications and they certainly contribute to the prevention of a material’s deterioration and to the improvement of the environment and quality of life.
For these reasons, ISO/TC 38/WG 23 developed ISO 20743 in 2007, and is continuing to study a test method on the antifungal activity of textile products for a series of International Standards.
This part of ISO 13629 adopts an ATP luminescence method as a basis for the quantitative determination of antifungal activity.
Characteristics of the luminescence method are as follows:
— extremely small margin of error compared to the colony count method;
— elimination of the culturing time for colony formation, enabling a shorter testing time;
— simplification of testing operation.
The other parts will be developed relating to:
— Part 2: Plate count method.
 
1   Scope
This part of ISO 13629 specifies a test method for the quantitative determination of the antifungal activity by measuring the intensity of luminescence produced by an enzymatic reaction [adenosine triphosphate (ATP) method].
The part of ISO 13629 is applicable to various kinds of textile products, such as fibres, yarns, fabrics, clothing, bedclothes, home furnishings and other miscellaneous goods.
Based on the intended application and on the environment in which the textile product is to be used, the user can select the most suitable evaluation method from the following methods before enumeration by the ATP method:
a) absorption method (an evaluation method in which test fungi suspension is inoculated directly onto the specimens);
b) transfer method (an evaluation method in which test fungi are placed on an agar plate and printed onto the specimens).
 
2   Normative reference
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 105-F02, Textiles — Tests for colour fastness —Part F02: Specification for cotton and viscose adjacent fabrics
 
3   Terms and definitions
For the purposes of this document, the following terms and definitions apply.
 
3.1 control specimen
specimen used to validate the growth condition of test fungus
Note 1 to entry: The control specimen may be taken from the same textile products as the textile products to be tested, but without antifungal treatment. If this is not available, a 100 % cotton specimen without fluorescent brighteners or other finish, complying with the requirements of ISO 105-F02, is used as the control specimen, after 10 cycles of washing for 10 min at a temperature of 60 °C without detergents or any brighteners and rinsing twice for 5 min in accordance with ISO 6330.
 
3.2 antifungal agent
agent to prevent or mitigate the growth of fungus or to reduce the number of fungus
 
3.3 antifungal treatment
treatment to prevent or mitigate the growth of fungus or to reduce the number of fungus
 
3.4 spore suspension
liquid with evenly dispersed fungal spores in sterilized water containing an anionic surfactant (8.3)
 
3.5 ATP
adenosine triphosphate, a multifunctional nucleotide present in living fungi
 
3.6 antifungal activity
activity to prevent or mitigate the growth of fungus, expressed as the difference of growth value in logarithm of ATP between the control and test specimen
 
3.7 luminescence method
method in which the amount of ATP contained in fungal cells is measured in moles of ATP
 

ISO 13629-2:2014 Textiles -- Determination of antifungal activity of textile products -- Part 2: Plate count method
 
ISO 13629-2:2014 specifies a test method for quantitative determination of antifungal activity by plate count method.
ISO 13629-2:2014 is applicable to various kinds of textile products such as fibres, yarns, fabrics, clothing, bedclothes, home furnishings, and other miscellaneous goods.
 
General information 
Status :  Published
Publication date : 2014-06
Edition : 1
Number of pages : 17
Technical Committee : ISO/TC 38 Textiles
ICS : 59.080.01 Textiles in general
 
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1.  In particular the different approval criteria needed for the different types of ISO documents should be noted.  This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.  Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISO's adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL:  Foreword - Supplementary information
The committee responsible for this document is ISO/TC 38, Textiles.
ISO 13629 consists of the following parts, under the general title Textiles — Determination of antifungal activity of textile products:
— Part 1: Luminescence method
— Part 2: Plate count method
 
Introduction
This part of ISO 13629 adopts the plate count method as a basis of quantitative determination of antifungal activity.
The following are characteristics of the plate count method:
— conventional method which is easy to operate in bacteriological laboratories;
— no need to use special apparatus such as a lumino photometer;
— long history and common procedure.
 
1   Scope
This part of ISO 13629 specifies a test method for quantitative determination of antifungal activity by plate count method.
This part of ISO 13629 is applicable to various kinds of textile products such as fibres, yarns, fabrics, clothing, bedclothes, home furnishings, and other miscellaneous goods.
 
2   Normative reference
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 105-F02, Textiles — Tests for colour fastness — Part F02: Specification for cotton and viscose adjacent fabrics
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
 
3   Terms and definitions
For the purposes of this document, the following terms and definitions apply.
 
3.1 control fabric
fabric used to validate the growth condition of test fungi
Note 1 to entry: Control specimens are sampled from the control fabric.
Note 2 to entry: The control fabric may be the same fabric as the fabric to be tested but without antifungal treatment. If this is not available, a 100 % cotton fabric without fluorescent brighteners or other finish, complying with the requirements of ISO 105‑F02, is used as control fabric, after washing at the temperature of 60 °C without detergents or any brighteners, with mechanical agitation and rinsing.
 
3.2 antifungal agent
chemical agent to prevent or mitigate the growth of fungi or to reduce the number of fungi
 
3.3 antifungal treatment
treatment to prevent or mitigate the growth of fungi or to reduce the number of fungi
 
3.4 spore suspension
liquid with evenly dispersed fungal spores in sterilized water containing an anionic surfactant
 
3.5 plate count method
method in which the number of fungi present after incubation is calculated by counting the number of colonies according to a ten-time dilution method
Note 1 to entry: The results are expressed in CFU (Colony Forming Unit).
 
3.6 neutralizer
chemical agent used to inactivate, neutralize, or quench the antifungal properties of antifungal agents
 
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