纺织品抗真菌活性测定 第2部分 平板法

时间:2015-05-05 09:02:42 来源: 点击量:

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ISO 13629-2:2014
Textiles — Determination of antifungal activity of textile products — Part 2: Plate count method
 
Introduction
This part of ISO 13629 adopts the plate count method as a basis of quantitative determination of antifungal activity.
The following are characteristics of the plate count method:
— conventional method which is easy to operate in bacteriological laboratories;
— no need to use special apparatus such as a lumino photometer;
— long history and common procedure.
 
1   Scope
This part of ISO 13629 specifies a test method for quantitative determination of antifungal activity by plate count method.
This part of ISO 13629 is applicable to various kinds of textile products such as fibres, yarns, fabrics, clothing, bedclothes, home furnishings, and other miscellaneous goods.
 
2   Normative reference
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 105-F02, Textiles — Tests for colour fastness — Part F02: Specification for cotton and viscose adjacent fabrics
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
 
3   Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
control fabric
fabric used to validate the growth condition of test fungi
Note 1 to entry: Control specimens are sampled from the control fabric.
Note 2 to entry: The control fabric may be the same fabric as the fabric to be tested but without antifungal treatment. If this is not available, a 100 % cotton fabric without fluorescent brighteners or other finish, complying with the requirements of ISO 105‑F02, is used as control fabric, after washing at the temperature of 60 °C without detergents or any brighteners, with mechanical agitation and rinsing.
3.2
antifungal agent
chemical agent to prevent or mitigate the growth of fungi or to reduce the number of fungi
3.3
antifungal treatment
treatment to prevent or mitigate the growth of fungi or to reduce the number of fungi
3.4
spore suspension
liquid with evenly dispersed fungal spores in sterilized water containing an anionic surfactant
3.5
plate count method
method in which the number of fungi present after incubation is calculated by counting the number of colonies according to a ten-time dilution method
Note 1 to entry: The results are expressed in CFU (Colony Forming Unit).
3.6
neutralizer
chemical agent used to inactivate, neutralize, or quench the antifungal properties of antifungal agents
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